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PKD2-mNG is positioned in two rows along the distal region of Chlamydomonas cilia. TIRF video showing the organization of PKD2-mNG in two rows along the distal region of the cilium; PKD2-mNG was expressed in the pkd2 mutant. Images were acquired at 10 fps. To generate the final video, we converted the original image stack using the “walking average” command in ImageJ set to 10 frames. The video corresponds to Fig. 2 Bb and is displayed at 50 fps (5× speed).
