Table 1. Overview of the available template plasmids for PCR tagging.
| Tag | Type | Oligos | Markerless | Zeocin | Puromycin | Application |
|---|---|---|---|---|---|---|
| moxBFP | Fluorescent protein | M1/M2 | pMaCTag-01 | pMaCTag-Z01 | pMaCTag-P01 | Imaging |
| TagBFP | Fluorescent protein | M1/M2 | pMaCTag-02 | pMaCTag-Z02 | pMaCTag-P02 | Imaging |
| moxCerulean3 | Fluorescent protein | M1/M2 | pMaCTag-03 | pMaCTag-Z03 | pMaCTag-P03 | Imaging |
| mTFP1 | Fluorescent protein | M1/M2 | pMaCTag-04 | pMaCTag-Z04 | pMaCTag-P04 | Imaging |
| eGFP | Fluorescent protein | M1/M2 | pMaCTag-05 | pMaCTag-Z05 | pMaCTag-P05 | Imaging |
| sfGFP | Fluorescent protein | M1/M2 | pMaCTag-06 | pMaCTag-Z06 | pMaCTag-P06 | Imaging |
| mNeonGreen | Fluorescent protein | M1/M2 | pMaCTag-07 | pMaCTag-Z07 | pMaCTag-P07 | Imaging |
| mClover3 | Fluorescent protein | M1/M2 | pMaCTag-08 | pMaCTag-Z08 | pMaCTag-P08 | Imaging |
| mVenus | Fluorescent protein | M1/M2 | pMaCTag-09 | pMaCTag-Z09 | pMaCTag-P09 | Imaging |
| mKOk | Fluorescent protein | M1/M2 | pMaCTag-10 | pMaCTag-Z10 | pMaCTag-P10 | Imaging |
| mScarlet-i | Fluorescent protein | M1/M2 | pMaCTag-11 | pMaCTag-Z11 | pMaCTag-P11 | Imaging |
| mScarlet | Fluorescent protein | M1/M2 | pMaCTag-12 | pMaCTag-Z12 | pMaCTag-P12 | Imaging |
| mCherry | Fluorescent protein | M1/M2 | pMaCTag-13 | pMaCTag-Z13 | pMaCTag-P13 | Imaging |
| mNeptune2.5 | Fluorescent protein | M1/M2 | pMaCTag-14 | pMaCTag-Z14 | pMaCTag-P14 | Imaging |
| miRFP670 | Fluorescent protein | M1/M2 | pMaCTag-15 | pMaCTag-Z15 | pMaCTag-P15 | Imaging |
| Dronpa3 | Fluorescent protein | M1/M2 | pMaCTag-16 | pMaCTag-Z16 | pMaCTag-P16 | Imaging |
| mEos4b | Fluorescent protein | M1/M2 | pMaCTag-17 | pMaCTag-Z17 | pMaCTag-P17 | Imaging |
| mKeima8.5 | Fluorescent protein | M1/M2 | pMaCTag-18 | pMaCTag-Z18 | pMaCTag-P18 | Imaging |
| mCherry-mNeonGreen | Fluorescent protein | M1/M2 | pMaCTag-19 | pMaCTag-Z19 | pMaCTag-P19 | Imaging |
| 10xSunTag | Detection tag | M1/M2 | pMaCTag-20 | pMaCTag-Z20 | pMaCTag-P20 | Imaging |
| 24xSunTag | Detection tag | M1/M2 | pMaCTag-21 | pMaCTag-Z21 | pMaCTag-P21 | Imaging |
| SNAP | Chem. modification | M1/M2 | pMaCTag-22 | pMaCTag-Z22 | pMaCTag-P22 | Imaging |
| Halo | Chem. modification | M1/M2 | pMaCTag-23 | pMaCTag-Z23 | pMaCTag-P23 | Imaging |
| TAP | Affinity tag | M1/M2 | pMaCTag-24 | pMaCTag-Z24 | pMaCTag-P24 | PPIs |
| YFP-LID | Degron | M1/M2 | pMaCTag-25 | pMaCTag-Z25 | pMaCTag-P25 | Function |
| mAID-Clover | Degron | M1/M2 | pMaCTag-26 | pMaCTag-Z26 | pMaCTag-P26 | Function |
| 1xHA | Antibody epitope | M1/M2 | pMaCTag-27 | pMaCTag-Z27 | pMaCTag-P27 | Detection |
| 3xHA | Antibody epitope | M1/M2 | pMaCTag-28 | pMaCTag-Z28 | pMaCTag-P28 | Detection |
| Strep-II | Affinity tag | M1/M2 | pMaCTag-29 | pMaCTag-Z29 | pMaCTag-P29 | Detection |
| VSV-G | Antibody epitope | M1/M2 | pMaCTag-30 | pMaCTag-Z30 | pMaCTag-P30 | Detection |
| V5 | Antibody epitope | M1/M2 | pMaCTag-31 | pMaCTag-Z31 | pMaCTag-P31 | Detection |
| S-Tag | Bimolecular compl. | M1/M2 | pMaCTag-32 | pMaCTag-Z32 | pMaCTag-P32 | Detection |
| FLAG | Antibody epitope | M1/M2 | pMaCTag-33 | pMaCTag-Z33 | pMaCTag-P33 | Detection |
| 3xFLAG | Antibody epitope | M1/M2 | pMaCTag-34 | pMaCTag-Z34 | pMaCTag-P34 | Detection |
For additional information, see Table S2. PPIs, protein–protein interactions.