Skip to main content
. 2020 May 11;219(6):e201908192. doi: 10.1083/jcb.201908192

Figure 2.

Figure 2.

BRCA1 may function upstream of BRCA2 in the stalled fork repair pathway. (A and B) IF analysis and graph of RPA32 recruitment in U2OS cells transfected with control siRNA (siLuc) or siRNA for BRCA1 or BRCA2, or both BRCA1 and BRCA2. These cells were fixed 3 h after UV damage (30 J/m2). Scale bars in A indicate 10 µm. (C and D) IF analysis and graph of RPA32 recruitment in U2OS cells transfected with control siRNA (siLuc) or siRNA for BRCA1, BRCA2, or both BRCA1 and BRCA2. Cells were collected 4 h after HU treatment (5 mM). Scale bars in C indicate 10 µm. Error bars indicate SD between triplicates. (E) Western blot analysis of nuclear extracts. RPA32 accumulation in U2OS cells transfected with indicated siRNAs was analyzed. Cells were treated with 5 mM HU and harvested 3 h after treatment. Nuclear extracts were prepared.