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. 2020 Apr 1;219(6):e201907082. doi: 10.1083/jcb.201907082

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© 2020 Jackman et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 5. — MAD2 recruitment to kinetochores is delayed when MAD1 cannot bind cyclin B1. (A) Maximum intensity projections at the indicated times from time-lapse fluorescence of parental RPE cyclin B1-Venus^+/−:Ruby-MAD2^+/−:RFP670-MIS12^+/+ cells and the MAD1 E53/E56K^+/+: RFP670-MIS12^+/+ clones 7D2 and 8B12 showing Ruby-MAD2 localization relative to NEBD. Scale bar, 5 µm. (B) Quantification of Ruby-MAD2 colocalization with RFP670-MIS12 using the Coloc-3DT program (see Materials and methods) relative to NEBD. Graphs show values obtained from at least 40 cells for each clone in three independent experiments. Error bars indicate SEM.