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. 2020 Apr 1;219(6):e201907082. doi: 10.1083/jcb.201907082

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© 2020 Jackman et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S2. — MAD1 recruits cyclin B1 to kinetochores. Related to Fig. 2. (A) Schematic showing guide RNA (gRNA, red) selection and protospacer adjacent motif (blue) for CRISPR-Cas9^D10A targeting of MAD1 exon4. (B) Genomic DNA sequencing of RPE cyclin B1-Venus^+/−:MAD2-Ruby^+/− MAD1 E53/56K clones 7D2 and 8B12.