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. 2020 Apr 1;219(6):e201907082. doi: 10.1083/jcb.201907082

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© 2020 Jackman et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S3. — MAD1 binding to cyclin B1 is required for genomic stability. Related to Fig. 3. The duration of the mitotic arrest for parental RPE cyclin B1-Venus^+/−:Ruby-MAD2^+/− cells and the MAD1 E53/E56K clones 7D2 and 8B12 was assayed by time-lapse DIC microscopy in 100 nM paclitaxel and plotted as black dots. The times in mitosis of cells that remained arrested for the duration of the experiment are shown separately as red dots. Total number of cells for each clone analyzed; parental n = 187 cells, clone 7D2 n = 201 cells, clone 8B12 n = 182 cells.