Figure S4.

Cells with MAD1 mutants that cannot bind cyclin B1 are sensitive to partial inhibition of MPS1. Related to Fig. 4. (A) Time from NEBD-anaphase for parental RPE cyclin B1-Venus^+/−:MAD2-Ruby^+/− cells and MAD1 E53/56K clones 7D2 and 8B12 treated with 0.62 µM AZ3146 MPS1 kinase inhibitor. Scatter dot pots show the median (gray line) from two independent experiments (parental n = 112 cells, 7D2 n = 111 cells, 8B12 n = 117 cells). The two-way P values were calculated using a Mann–Whitney unpaired t test.