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Spinning disk confocal video shows mitotic entry of MAD1 E53/E56K clones 7D2. Cyclin B1-Venus (far left panel), Ruby-MAD2 (left panel, green), RFP670-MIS12 (right panel, red), and merged channels for MAD2 and MIS12 (far right panel). Cells treated with reversine (166 nM) just before imaging. Frame rate, one image every 2 min.
