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Projected Z-series stacks of cells filmed using confocal spin disk microscopy with 2-min time frame. Parental RPE cyclin B1-Venus+/−:Ruby-MAD2+/−:RFP670-MIS12+/+ expressing POM121-GBP-mTurq2. Panels, left to right, show cyclin B1-YFP, Mad2-Ruby, RFP670-Mis12, and then merged image. Merged image Mad2-Ruby (green), RFP670-Mis12 (red).
