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. 2020 Jun 2;11:2777. doi: 10.1038/s41467-020-16605-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Green: Type IV collagen α5 chain. Red: WT1 as a podocyte marker. a Wild-type mouse treated with the vehicle. b Mutant mouse treated with the vehicle. c–f Mutant mouse treated with the antisense oligonucleotide (ASO) (e, f cropped). Type IV collagen α5 chain was completely negative in the vehicle group (b), but was clearly expressed on the tubular basement membrane and even on the glomerular basement membrane (GBM); although, expression was only partial on the GBM (e, f arrows). Scale bars are 100 μm. Two different researchers from different institutes conducted the staining, and the expression pattern was identical.