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. 2020 Jun 2;10:8936. doi: 10.1038/s41598-020-65823-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of Phase II Temperature Increase: The first temperature increasing phase (Phase I, 25 °C → 37 °C) could cause significant elevation of apoptotic rate. (A) The heating protocols. I/E + II-III/WB: Phase I was heated by mEHT and Phase II and III were heated by WB. I/E + II-III/37 °C: Phase I was heated by mEHT and Phase II and III were shifted to 37 °C incubator. (B) Total number of corresponding Joules emitted per each experiment was recorded and shown as bar chart as previous figure described. (C) Apoptosis was measured as previous figure described. Histograms for the percentage of Annexin-V-positive cells are shown. Data represent results from 3 independent experiments; bars indicate mean ± standard deviation (SD). (*P < 0.05).