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. 2020 Jun 2;11(6):412. doi: 10.1038/s41419-020-2617-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a qRT-PCR analysis showed that RUNX1-IT1 expression was knocked down in PANC-1, CFPAC-1 and SW1990 cells by Smart Silencer lncRNA (RUNX1 Silencer and Control Silencer). b CCK8 assays were used to assess the viability of two groups of PC cells transfected with either RUNX1-IT1 Silencer or Control Silencer. c, d EdU assays were used to assess the cell proliferation ability. e, f Migration ability was assessed by a wound healing assay. g, h Transwell assays were used to assess the cell migration and invasion abilities. i Abdominal MRI scans of nude mice after 6 weeks of orthotopic PC xenograft growth. The livers of nude mice in the RUNX1-IT1 knockout PANC-1 cell group showed few metastatic lesions, but obvious metastatic lesions were found in the livers of the control group mice (CRISPR-Cas9 lentiviral system). j Images of micrometastases on the liver surface in the RUNX1-IT1 knockout PANC-1 group and in the control group. k HE staining of liver metastatic lesions was performed. The representative HE images are shown (scale bars, 1000 and 100 μm). l Histogram indicating the numbers of metastasized lesions in the two groups of mice. The number of metastatic lesions in the RUNX1-IT1 knockout PANC-1 group (N = 5) was significantly decreased compared with that in the control group (N = 5) (*P < 0.05, **P < 0.01, ***P < 0.001).