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. 2020 Jun 2;11(6):412. doi: 10.1038/s41419-020-2617-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Heatmap of differentially expressed downstream genes in RUNX1 and RUNX1-IT1 knockdown PANC-1 cells and the corresponding control cells (P < 0.05, fold change > 2). b Top 30 enriched pathways in RUNX1 and RUNX1-IT1 knockdown PANC-1 cells. c GSEA analysis of the RUNX1 and RUNX1-IT1 knockdown PANC-1 groups and the control. d The levels of downstream TNF genes in the RUNX1 and RUNX1-IT1 knockdown PANC-1 groups were analyzed by qRT-PCR and compared with those in the control group. e The Venn diagram shows three targets in the area common to the four groups, namely, RUNX1-IT1 downstream genes, RUNX1 downstream genes (fold change > 2, P < 0.05), RUNX1-IT1 TNF core enriched genes and RUNX1 TNF core enriched genes (P < 0.05). f Correlation analysis between C-FOS and RUNX1 and between C-FOS and RUNX1-IT1 in GEO (GSE15471). g, h The C-FOS gene expression levels in the RUNX1-IT1 knockdown and RUNX1 knockdown groups were analyzed by qPCR. i The C-FOS gene expression levels in the NC, ex-RUNX1-IT1 and ex-RUNX1-IT1+sh-RUNX1 groups were analyzed by qRT-PCR. j The protein expression levels of C-FOS-related downstream molecules in the C-FOS knockdown PC and control cells were assessed by WB. k, l The protein expression levels of C-FOS and its related downstream molecules in the RUNX1-IT1 knockdown and RUNX1 knockdown groups were assessed by WB. m The protein expression levels of C-FOS and its related downstream molecules in the NC, ex-RUNX1-IT1 and ex-RUNX1-IT1 + sh-RUNX1 groups were assessed by WB (*P < 0.05, **P < 0.01, ***P < 0.001).