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. 2020 Jun 2;10:8943. doi: 10.1038/s41598-020-65696-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Average pK_a determination of SsPDO. rSsPDO (1 µM) was reacted with CDNB (1 mM) at variable pH values (red line). Average pK_a = 8.6 ± 0.1 of the three reactive cysteines in rSsPDO. As a control experiment, GSH (100 µM) was reacted with CDNB (1 mM) at variable pH values (gray line). The error bars represent the S.D. from three independent experiments. The theoretical curve (black line) of an unperturbed protein cysteine (pK_a = 9.1) is also reported.