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. 2020 Jun 2;10:8968. doi: 10.1038/s41598-020-65933-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of indirect and inhibitory ELISA methods for determination of anti-IFN-γ autoantibody. Anti-IFN-γ autoantibody titers were measured from heparinized plasma samples by indirect and inhibitory ELISA. A scatter dot plot presents the absorbance index of indirect ELISA from NTM infection patients (inhibitory ELISA positive n = 76, and negative n = 6), lymphadenopathy without infection (n = 20), and non-infected controls (n = 20). The dashed line represents the diagnosis cut-off. Statistically significant differences were further analyzed using ANOVA (Kruskal-Wallis test) with Dunn’s multiple comparisons post-test, ***P < 0.001 and ****P < 0.0001 (A). Correlation of positive results (n = 68) between the Log₁₀ absorbance index from indirect ELISA was compared to Log₁₀ titer from inhibitory ELISA using linear regression (B).