Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 2;10:8968. doi: 10.1038/s41598-020-65933-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Plasma anti-IFN-γ autoantibodies identified by indirect and/or inhibitory ELISA neutralized phosphorylation of STAT1. Recombinant human IFN-γ 200 ng/ml was pre-incubated with each plasma sample at a 1:10 dilution before being cultured with 10⁴ CD14-FITC-labeled human PBMCs for 30 min. Phosphorylation of STAT1 (pSTAT1) was stained intracellularly and analyzed by flow-cytometer. Representative histograms of pSTAT1 signal of CD14 positive cells compared with no stimulation (dotted line) and IFN-γ stimulation (tinted black line) are shown (A). Median fluorescent intensity (MFI) from each sample from different groups was calculated as % pSTAT1 MFI stimulation index = (MFI _{plasma + IFN-γ} – MFI _unstimulated/MFI _IFN-γ – MFI _unstimulated) × 100. A scatter dot plot of % pSTAT1 MFI stimulation index of 5 plasma samples from each group shows the median line and interquartile range (B).