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. 2020 Jun 2;10:8968. doi: 10.1038/s41598-020-65933-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution of anti-IFN-γ autoantibody titers of NTM infected patients. Anti-IFN-γ autoantibody titers were measured in heparinized plasma samples by inhibitory ELISA. The results are presented as a scatter dot plot and bar graph plot with the geometrical mean and 95%CI. Statistically significant differences among each sample group—of: duration of NTM infection (A); type of infection (B); and, NTM infection outcomes (C)—were compared using an ANOVA (Kruskal-Wallis test) with Dunn’s multiple comparisons post-test, *P < 0.05, **P < 0.01 and ****P < 0.0001. Anti-IFN-γ autoantibody titers of active NTM infected patients (n = 14) were followed-up for 3 years; the results of which are presented as a scatter dot plot with connecting line for the same patient samples (D). Black lines represent reduction of titers, and dash lines are non-changing titers. Statistically significant change of titers from repeated samples were analyzed using an ANOVA (Friedman test).