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. 2020 Jun 2;10:9002. doi: 10.1038/s41598-020-66050-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of the dbToxRp construct showing it is similar in structure to ToxRp, destabilized by bile salts, and can bind ToxSp. (a) A HSQC of ¹⁵N-dbToxRp in black overlaid with a HSQC of ¹⁵N-ToxRp in red. The NMR data was collected on a Bruker 700 MHz instrument. (b) Melting temperatures of dbToxRp when treated with 2 mM cholate and chenodeoxycholate as measured by DSF. Each trial is graphed as well as the mean and SD, n = 3. (c) A colloidal blue stained gel showing the dbToxRp can be pulled down by CBD-ToxSp. Lane 1–3: serial dilutions 1×-1/4x of the CBD-ToxSp pull down with dbToxRP, Lane 4: dbToxRp with beads only.