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. 2020 Jun 2;11:2772. doi: 10.1038/s41467-020-16497-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a The transsulfuration pathway showing one of the reactions catalyzed by the CBS enzyme. b ELISA determination of hCys levels in PA-CF and TI-CF relative to SF at 40 hpf. Box plots with superimposed data points of 5–7 biological replicates. Graph represents box and whiskers min to max. Asterisks: p = 0.001 and 0.003 from left to right. Significance was determined by unpaired Student’s t test. c Expression of atf3 during PA-CF, TI-CF, and SF development determined by qualitative RT-PCR. Three biological replicates with similar results. d–h Effects on eye development at 4 dpf after injection of 5 mM hCys into SF eggs (f). d, e Normal eyes in un-injected (UI) SF eggs (d) and SF eggs injected with 5 mM cysteine (Cys) (e). f–h Abnormal eyes with a hemorrhage (arrow) (f) or with a ventrally displaced lens (arrow) (g), and complete suppression of eye formation (h) in SF eggs injected with 5 mM hCys. SF in f was treated with PTU to visualize hemorrhage. Scale bar: 200 μm; same magnification in d–h. n values: see Supplementary Table 2. Source data are provided in source data file.