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. 2020 Jun 2;11:2765. doi: 10.1038/s41467-020-16533-w

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Co-expression of pri-miR-27a and FLAG-AGO2 constructs: wild-type (WT), PAZ mutant (F2L3), and slicer-dead (D597A). Detection of miR-27a-3p in input and FLAG-immunoprecipitate (IP) by Northern blot. b Sequence composition of miR-27a-3p reads bound to AGO2-WT and AGO2-F2L3 on IGV (integrative genomics viewer). c Co-expression of FLAG-AGO2 constructs and miRNAs with preferential loading of 3p and 5p arms (miR-23a-3p, miR-1-3p, miR-122-5p, and miR-15-5p). Detection of mature miRNA in input and FLAG-immunoprecipitate (IP) by Northern blot. Source data are provided as a Source Data file.