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. 2020 Jun 2;11:2765. doi: 10.1038/s41467-020-16533-w

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2020

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Fig. 3 — a Co-expression of pri-miR-27a and FLAG-AGO2 constructs: wild-type (WT) and PAZ domain deletion (ΔPAZ). Detection of miR-27a-3p in input and FLAG-immunoprecipitate (IP) by Northern blot. b Nucleotide composition of miR-27a-3p oligo-tail. Horizontal axis indicates the absolute number of non-templated U nucleotides in tail, and the vertical axis indicates the cumulative percentage from the longest to the shortest oligo-tails. c, d Co-expression of pri-miR-27a and AGO2-ΔPAZ (c) or AGO2-F2L3 (d) in wild-type HEK293T or TUT4/7 DKO, and TUT4/7 DKO cells rescued with either GFP, TUT4, TUT7, or TUT4 and TUT7. Detection of miR-27a-3p by Northern blot. Source data are provided as a Source Data file.