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. 2020 Jun 2;11:2765. doi: 10.1038/s41467-020-16533-w

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2020

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Fig. 6 — a Scatter plot of U-score (change in average number of oligo-U between DIS3L2 KO and wild-type cells, y-axis) and expression of endogenous miRNA (in counts per million, x-axis). Highlighted in blue, miR-7-5p, miR-222-3p, miR-769-5p, and miR-21-5p (control miRNA). b Comparing the composition of oligo-tail (number of U or A in the tail, x-axis) and cumulative percentage (y-axis) of miR-7-5p, miR-222-3p, and miR-769-5p, in wild-type, TUT4/7 DKO and DIS3L2 KO cells. c Detection of tailing of endogenous miR-7-5p, miR-222-3p, and miR-769-5p in wild-type, TUT4/7 DKO, DIS3L2 KO, and TUT4/7/DIS3L2 Triple KO (TKO) by Northern blot. miR-21-5p was also detected as a control. d Northern blot detecting endogenous miR-7-5p and miR-21-5p (control miRNA) in HEK293T cells upon knockdown or overexpression of CYRANO by siRNA (two independent sequences) or plasmids with miR-7 binding site (CYRANO) or mutated site (CYRANO-mut). e Northern blot detecting endogenous miR-7-5p and miR-21-5p (control miRNA) in wild-type, TUT4/7 DKO and DIS3L2 KO cells transfected with plasmids expressing CYRANO or CYRANO-mut. Source data are provided as a Source Data file.