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. 2020 Jun 2;11(6):414. doi: 10.1038/s41419-020-2612-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Schematic representation of the design of in vivo experiments. b Representative 3D μCT images of reconstructed mouse calvarial (from internal to external/from external to internal). c Quantification of trabecular number (Tb. N), trabecular separation (Tb. Sp), trabecular thickness (Tb.Th), and trabecular bone volume fraction (BV/TV). d Representative images of cranial sections stained with H&E, Masson, and TRAP from each group. Scale bars=200μm. e Quantification of percentage of bone area in the border zone of the cranial H&E staining by using Image J software and the analysis of TRAP-positive cells in each group. f ELISA to detect the concentration of IL-1β, IL-6, and TNF-α in serum. The data in the figures represent the averages±SD. N.S. represented as no significant difference. Significant differences are indicated as *p<0.05 or **p<0.01 paired using Student’s t-test unless otherwise specified.