Fig. 4. Combo induces neutrophil turnover and reduces their prevalence.

a Old C57BL/6 mice (>20 weeks) were treated with isotype control antibody (Ctr), anti-Ly6G or the combination strategy (Combo) for 18 days. 60 h before sampling, mice were injected once with 1 mg of BrdU intraperitoneally. b Neutrophil prevalence among all immune cells was assessed in bone marrow, blood, spleen and lung while the impact on their dynamic flow rate was measured with BrdU-60h labelling. Both anti-Ly6G and Combo treatment elicit a shift of BrdU neutrophils from the bone marrow stock to the peripheral tissue, but only the Combo treatment is “fast” enough to also reduce neutrophil prevalence. BM, bone marrow. c,d Validation of protocol efficacy with Trucount flow cytometry analysis in (c) blood and (d) lung tissue. e Scheme representing neutrophil counts in the circulation from a dynamic perspective, with the impact of increased flow rate on the global prevalence of neutrophils depending on the killing celerity of the depletion strategy. *p < 0.05, **p < 0.01 from Mann–Whitney test; error bars represent s.d. b n = 7 control (Ctr) and 5 treated mice per group. c,d n = 5 mice per group. Source data are provided as a Source Data file.