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. 2020 Jun 2;11:2767. doi: 10.1038/s41467-020-16525-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Representative western blot of homogenized dissected intestinal segments from WT mice (duodenum n = 3; jejunum n = 4; ileum and colon n = 5) showed greatest ABCG2 abundance in the small intestine jejunum and ileum segments; beta-actin used as loading control; summary data of ABCG2 abundance. b Representative immunofluorescence micrographs of fixed sections of mouse small intestines stained for ABCG2 (green), villi brush border marker beta-actin (red), and colocalization of ABCG2 and beta-actin (yellow) at 200× (all scale bars 10 μM) and 400x or c stained for SLC2A9 (green), Na+/K+ ATPase (red), DAPI (blue), and colocalization of SLC2A9 and Na+/K+ ATPase (yellow) 400×, with Z projections (all scale bars 10 μM) (b, c representative images of sections from n = 4 mice). Source data are provided as a Source data file.