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. 2020 Jun 2;11(6):415. doi: 10.1038/s41419-020-2626-6

Fig. 1. TNF-α-induced overexpression of pro-inflammatory genes, through activation of NF-kB pathway in microglia.

Fig. 1

Rat microglia were obtained from mixed glial cultures. Isolated microglia were re-seeded in six-well plates, allowed to adhere for 48 h and then stimulated for 6 h with LPS (100 ng/mL) or TNF-α (20 ng/mL). a Gene expression profile of activated microglia evaluated by RT-qPCR. Results were normalized with Gapdh and are expressed in fold change to CTR (mean ± SD, n = 5). b NF-kB p65 phosphorylation levels evaluation by western blot after microglia activation with LPS or TNF-α (mean ± SD, n = 5). GAPDH was used as normalizer. Statistical significance: **p < 0.01, *p < 0.05, ns–non significant; Friedman test followed by Dunn’s multiple comparisons test.