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[Preprint]. 2020 May 28:2020.05.27.120204. [Version 1] doi: 10.1101/2020.05.27.120204

Figure 2.

Figure 2.

Representative IMAC chromatography fraction analyses and purified proteins. M – protein standards, molecular weights of select standards noted in kDa. A. Coomassie-stained SDS-PAGE analysis of standard IMAC protocol (VRC spike), S – culture supernatant, FS – filtered culture supernatant, P – TFF permeate, L – TFF retentate/column load, FT – column flow through, W – column wash. Fractions pooled are underlined. B. Coomassie-stained SDS-PAGE analysis of representative purified proteins. C. Representative Coomassie-stained SDS-PAGE analysis of Ni magnetic bead chromatography and final protein (VRC spike). FS – filtered culture supernatant, U – unbound protein.