Table 1.

Effect of expression and purification parameters on spike yield. Unless noted, the post-harvest production process followed the procedure of TFF > IMAC > desalting column. Numbers represent yields from independent experiments. nd - not determined.

0BCondition	1BVRC (mg/l)	2BMt. Sinai (mg/l)
3B37°C/72 hr IMAC/SEC	0.3	0.9
4B37°C/96 hr IMAC/desalt	2.0, 2.0, 1.4	1.7, 2.6
5B32°C/96 hr IMAC/desalt	4.8, 5.2, 6.1	6B4.6, 5.3
7B32°C/120 hr IMAC/desalt	6.4, 5.0	8B4.4
9B32°C/96 hr MagBeads/desalt	4.1, 5.5	10Bnd