Fig. 8.

FGF2 protects against cisplatin-induced cell apoptosis via the Erk1/2 pathway in NRK-52E cells. a Western blot results showing the induction of p-Erk1/2 in a time-dependent manner. NRK-52E cells were treated with FGF2 (10 ng/mL) for various duration as indicated. b Mek1 inhibitor (PD98059, 10 µM) effectively blocked FGF2-induced Erk-1, 2 signaling activation. c Representative immunofluorescent staining images for cleaved caspase 3 showing that FGF2 protein could prevent cisplatin-induced apoptosis in NRK-52E cells. NRK-52E cells were treated with cisplatin (25 µg/mL) for 12 h in the absence or presence of FGF2. Blockade of Erk1/2 signaling with PD98059 partly diminished the protective effect of FGF2 on cisplatin-induced cell apoptosis. Coverslips were counterstained with DAPI for nuclear visualization. d Quantitative determination of cleaved caspase 3-positive cells among groups as indicated. Data are presented as the percentage of the cells. * p < 0.05, n = 4.