(A) Cell viability after treatment with Cordyceps
militaris for 24 hours, 48 hours, and 72 hours. (B)
Detection of morphological changes by C militaris
treatment for 48 hours and 72 hours. Cordyceps
militaris dose- and time-dependently inhibits cell
proliferation and induces morphological changes in A549 cells (non–small
lung cancer cells). (A) Inhibition of the growth of A549 cells by
C militaris. A549 were exposed to 0 (vesicle), 25,
50, 100, 200, 500, and 1000 µg/mL C militaris extract
for 24 hours, 48 hours, and 72 hours prior to estimation of cell number
using the CCK-8 assay. The experiment was performed in triplicate.
C militaris significantly inhibited cell
proliferation of A549 cells. (B) Morphological changes of A549 cells
treated with C militaris compared with control
(vehicle). Microscopic images of A549 treated with C
militaris for 48 hours and 72 hours. Magnification ×100 and
×400. The statistics demonstrated that the percentage of the cells
mainly represents treated cells, which was apparent when the percentage
of control cells markedly decreased. Data are presented as means ±
standard deviations from triplicate experiments. Statistical
significance was considered as ***P < .001 versus
vehicle treatment; ns, nonsignificance.