Fig. 4.
Inhibition of PI3K/AKT leads to an accumulation of p27Kip1 in the nucleus in renal cell carcinoma (RCC) cells. a Immunofluorescence microscopic analysis of A-498 cells for p27Kip1 after treatment with 20 μM Ly294002 or solvent control for 48 h. Nuclei stained with 4′,6-diamidino-2-phenylindole (DAPI). Scale bar = 50 μm. b Quantification of the proportion of cells with nuclear p27Kip1 expression after treatment described in a. Each bar represents mean and standard deviation of 3 independent experiments (* p < 0.05).