Fig. 3.

Monoclonal IgG anti-Ara h 2 protects against systemic and local peanut allergy in a FcγRIIb-dependent manner. a Temperature after i.v. challenge with peanut extract of peanut-sensitized mice to assess anaphylaxis was measured rectally every 10 min for 50 min. Mice were pretreated 24 h before challenge with monoclonal IgG anti-Ara h 2 or an isotype control IgG. b Effect of IgG anti-Ara h 2 and involvement of FcγRIIb was addressed on a local level via skin prick test. FcγRIIb was locally blocked with DARPin. Means ± SEM are shown (n = 3 mice per group, data are representative of 3 independent experiments). Statistical significance was analyzed by two-tailed t test. c To address FcγRIIb involvement in protection, mice received 150 µg of anti-FcγRIIb or an isotype control IgG 24 h before challenge. Means ± SEM are shown (n = 4 to 5 mice per group). Data are representative of 3 independent experiments. Statistical significance was analyzed by two-way ANOVA test.