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. 2020 Jun 2;246:108748. doi: 10.1016/j.vetmic.2020.108748

Fig. 4.

Fig. 4

Specific inhibition of ICAM1 decreased Pasteurella multocida (PM) adherence to bTECs. (A) Bar graph representing bTEC treatment with increasing concentrations of anti-ICAM1 antibody and incubation with PM. The degree of PM adherence to the cells was evaluated by counting PM-positive colonies. The data represent n = 3; error bars: SEM; *p < 0.05. (B) FITC-labeled PM (green) adherence to the antibody-treated cells was observed with a fluorescence microscope. An isotype control antibody was used as control. The nuclei were stained with Hoechst-33342 (blue) (scale bar: 30 μm).