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. 2020 Jun 2;246:108748. doi: 10.1016/j.vetmic.2020.108748

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Fig. 5 — Knockdown of ICAM1 decreased Pasteurella multocida (PM) adherence to bTECs. (A) bTECs were transfected with siRNA targeting ICAM1 (ICAM1-A and ICAM1-B), and ICAM1 expression was evaluated by western blot. (B) The relative ICAM1 expression level was calculated by dividing the band density of ICAM1 by that of the GAPDH loading control. The data represent n = 3; error bars: SEM; *p < 0.05. (C) The degree of PM adherence to bTECs was evaluated by counting PM-positive colonies. The data represent n = 3; error bars: SEM; *p < 0.05. (D) FITC-labeled PM (green) adherence to the siRNA-transfected cells (ICAM1-A) was observed with a fluorescence microscope. The nuclei were stained with Hoechst-33342 (blue) (scale bar: 30 μm).