TABLE 3.

Feeding of industrial fermented P. pastoris KM71H-tmfA to Ae. aegypti Larvae.

Number of cells/ml	TMOF (nM)	Feeding days	Mortality (%) ± SEM
1.65 × 108	229	10	100
8.27 × 107	114	8	100
4.97 × 107	57	10	25 ± 3
1.6 × 108 (control)	0	10	0

Ae. aegypti larvae 24 h after emergence, 16 groups (5 larvae per group) were fed in tissue culture wells containing 25 ml of sterile distilled water different amounts of recombinant P. pastoris KM71H-tmfA cells for 10 days. The yeast cells were fermented in 150 L fermenter for 120 h and were heat treated in oven. Larval mortality was daily followed up to 10 days. P. pastoris cells that were not genetically engineered were used as control.