Table 2.
PCR components and setup.
| Platinum™ Taq (10 units/μl) | Taq2000™ (5 units/μl) | DreamTaq™ (5 units/μl) | TaKaRa Ex Taq™ (5 units/μl) or combined with DreamTaq™ (5 units/μl) | PicoMaxx™ System (5 units/μl) or combined with DreamTaq™ (5 units/μl) | pfu DNA polymerase (2.5 units/μl) or combined with DreamTaq™ (5 units/μl) | Herculase® II Fusion DNA polymerase | Phusion™ High-Fidelity DNA Polymerase (2 units/μl) | Pwo DNA polymerase (5 units/μl) or combined with DreamTaq™ (5 units/μl) | |
|---|---|---|---|---|---|---|---|---|---|
| Water (μl) | Mixture A: 7.6 | ||||||||
| Water (μl) | 15.375 | 16 | 16.375/16.25 | 15.875 or 15.75 | 17.3 or 17.175 | 17.05/17.55 or 16.925/17.425 | 16 | 14.5 | Mixture B: 9.875 (or 9.75) |
| 10 or 5x proprietary buffer (μl) | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 | 5 | 5 | Mixture A: 2.5 |
| 50 mM MgCl2 (μl) | 1 | 0.25 | |||||||
| 100 mM dNTP (25 mM each) (μl) | 0.2 | 0.2 | 0.25 | Mixture B: 0.4 | |||||
| 10 mM dNTP (2.5 mM each) (μl) | 2 | 0.5 | |||||||
| 8 mM dNTP (2 mM each) (μl) | 2.5 | 2.5 | 2.5 | ||||||
| 10 μm Forward primer (μl) | 0.75 | 0.75 | 0.75 | 1.25 | 1.25 | 1.25 | 0.625 | 1.25 | Mixture B: 1.25 |
| 10 μm Reverse primer (μl) | 0.75 | 0.75 | 0.75 | 1.25 | 1.25 | 1.25 | 0.625 | 1.25 | Mixture B: 1.25 |
| DMSO | 0.25 | ||||||||
| DNA template (μl) | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | Mixture B: 2 |
| Proprietary DNA polymerase(s) (μl) | 0.125 | 0.25 | 0.125/0.25 | 0.125 or plus DreamTaq™: 0.125 | 0.5 or plus DreamTaq™: 0.125 | 0.75/0.25 or plus DreamTaq™: 0.125 | 0.5 | 0.25 | Mixture A: 0.125 or plus DreamTaq™: 0.125 |
| Total reaction volume (μl) | 25 | 25 | 25 | 25 | 25 | 25 | 25 | 25 | 12.5 each mixture |