Table 3.

PCR cycling conditions.

	Platinum™ Taq		Taq2000™		DreamTaq™		TaKaRa Ex Taq™ or combined with DreamTaq™		PicoMaxx™ System or combined with DreamTaq™
1. Initial denaturation	95°C for 3 min	Sep 1: 1 cycle	95°C for 3 min	Step 1: 1 cycle	95°C for 3 min	Step 1: 1 cycle	98°C for 30 sec	Step 1: 1 cycle	95°C for 2 min	Step 1: 1 cycle
2. Denaturation	95°C for 30 sec	Step 2, 3 and 4: 36 cycles	95°C for 30 sec	Step 2, 3 and 4: 36 cycles	95°C for 30 sec	Step 2, 3 and 4: 36 cycles	98°C for 10 sec	Step 2 and 4: 36 cycles	95°C for 30 sec	Step 2, 3 and 4: 36 cycles
3. Annealing	50°C for 45 sec		50°C for 45 sec		50°C for 45 sec				55°C for 45 sec
4. Extension	72°C for 3 min		72°C for 3 min		72°C for 3 min		68°C for 5 min		72°C for 5 min
5. Final extension	72°C for 7 min	Step 5: 1 cycle	72°C for 7 min	Step 5: 1 cycle	72°C for 7 min	Step 5: 1 cycle	72°C for 7 min	Step 5: 1 cycle	72°C for 7 min	Step 5: 1 cycle