Table 4.

PCR cycling conditions.

No. Step	pfu or combined with DreamTaq™		Herculase® II		Phusion™		Pwo or combined with DreamTaq™
1. Initial denaturation	95°C for 2 min	Step 1: 1 cycle	95°C for 2 min	Step 1: 1 cycle	95°C for 2 min	Step 1: 1 cycle	95°C for 2 min	Step 1: 1 cycle
2. Denaturation	95°C for 30 sec	Step 2, 3 and 4: 36 cycles	95°C for 20 sec	Step 2, 3 and 4: 36 cycles	95°C for 20 sec	Step 2, 3 and 4: 36 cycles	95°C for 30 sec	Step 2, 3 and 4: 36 cycles
3. Annealling	55°C for 45 sec		55°C for 20 sec		55°C for 20 sec		57°C for 45 sec
4. Extension	72°C for 5 min		72°C for 2 min 15 sec		72°C for 2 min 15 sec		72°C for 5 min
5. Final extension	72°C for 7 min	Step 5: 1 cycle	72°C for 7 min	Step 5: 1 cycle	72°C for 7 min	Step 5: 1 cycle	72°C for 7 min	Step 5: 1 cycle