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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Neurobiol Dis. 2020 Apr 10;140:104863. doi: 10.1016/j.nbd.2020.104863

Figure 4. Termination of SE by urethane promotes hippocampal neuroprotection 24 hours after SE onset.

Figure 4.

Representative images of FluoroJade B staining in hippocampal sections (40 μm) in the cornu ammonis 1 (CA1) region 24 h after DFP-induced status epilepticus for rats that experienced uninterrupted SE (A), rats treated with diazepam after 1 h of SE (B), and rats injected with urethane after 1 h of SE (C). The images were taken at 100x total magnification. The dashed circles and the arrowheads in the boxed inserts highlight pyramidal cells in CA1. The images are representative of 5 dorsal hippocampal sections per rat. Scale bar = 200 μm. D, the average number of injured neurons per section 24 hours after DFP-induced status epilepticus in three dorsal hippocampal regions (hilus, CA1 and CA3) of rats that experienced uninterrupted SE (white bar, open triangles, n = 7 rats), rats treated with diazepam following 1 h of SE (gray bar, open squares, n = 11 rats) and rats injected with urethane after 1 h of SE (black bar, open circles, n = 8 rats) (p = .0002 in CA1 and p = .002 in CA3, by Mann-Whitney test comparing urethane to diazepam). The bars show the mean and standard error of the mean. The symbols represent each individual rat within the group.