Table 2.
CT values and geometric means of mRNA for three housekeeping genes obtained from brain hemisphere in groups of rats for 24-hour inflammatory mediator measurement. The number in parentheses represents the number of rats in each group.
| Uninterrupted | β-Actin | GAPDH | HPRT1 | Geomean |
|---|---|---|---|---|
| Control (6) | 22.5 ± 0.3 | 21.3 ± 0.2 | 24.4 ± 0.1 | 22.7 ± 0.2 |
| DFP-SE (7) | 23.5 ± 0.2 | 20.4 ± 0.3 | 24.2 ± 0.1 | 22.6 ± 0.1 |
| Diazepam-1 hr | β-Actin | GAPDH | HPRT1 | Geomean |
| Control (4) | 20.4 ± 0.3 | 19.1 ± 0.3 | 22.5 ± 0.04 | 20.6 ± 0.2 |
| DFP-SE (9) | 18.3 ± 0.6 | 17.9 ± 0.6 | 21.7 ± 0.4 | 19.2 ± 0.5 |
| Urethane-1 hr | β-Actin | GAPDH | HPRT1 | Geomean |
| Control (5) | 22.1 ± 0.5 | 20.7 ± 0.5 | 23.6 ± 0.6 | 22.1 ± 0.5 |
| DFP-SE (11) | 21.7 ± 0.3 | 20.9 ± 0.3 | 23.7 ± 0.3 | 22.1 ± 0.3 |
Data are the mean ± standard error