Table 4.

Estimation statistics for qRT-PCR mRNA fold change from brain hemispheres with 5000 bootstrap iterations

Analyte/treatment	Mean ± SEM	Mean difference (compared to urethane)	95% confidence interval	p-value
IL-1β
Uninterrupted (7)	287 ± 53	284.9	199 – 389	0.0006
Diazepam (9)	10 ± 2	8.3	5.5 – 13.8	0.0003
Urethane (11)	2 ± 0.4	0	-	-
TNFα
Uninterrupted (7)	2 ± 0.5	−0.7	−1.5 – 0.7	0.15
Diazepam (9)	2.9 ± 0.6	0.4	−0.6 – 1.7	0.94
Urethane (11)	2.6 ± 0.2	0	−	−
IL-6
Uninterrupted	16 ± 3.3	2.8	−5.4 – 11.9	0.5
Diazepam	31 ± 3.4	17.8	7 – 25	0.006
Urethane	13 ± 3.2	0	-	-
COX-2
Uninterrupted	8 ± 0.7	1.9	−0.4 – 4.3	0.15
Diazepam	13.4 ± 1.6	7.5	3.8 – 11	0.003
Urethane	5.9 ± 1	0	-	-
cFos
Uninterrupted	8 ± 1.5	3.3	0.4 – 7.3	0.02
Diazepam	5.6 ± 1.5	1.2	−1.6 – 5.2	0.65
Urethane	4.4 ± 1	0	-	-
NGFI-c
Uninterrupted	8 ± 2.4	5.7	2.7 – 12.9	0.004
Diazepam	5.5 ± 1.5	3.3	0.9 – 7	0.06
Urethane	2 ± 0.4	0	-	-
CCL2
Uninterrupted	1040±113	754	453 – 980	0.002
Diazepam	604 ± 69	320	62 – 490	0.003
Urethane	284 ± 85	0	-	-
CCL3
Uninterrupted	13 ± 1.5	11.1	8.1 – 13.7	0.0006
Diazepam	13.6 ± 3.2	12.1	7.7 – 21	0.0002
Urethane	1.5 ± 0.2	0	-	-
CCL4
Uninterrupted	63 ± 21	61.2	33.5 – 118	0.0006
Diazepam	7 ± 2	5.1	2.3 – 10.3	0.003
Urethane	1.9 ± 0.4	0	-	-
TGFβ1
Uninterrupted	1.3 ± 0.2	−0.9	−1.8 – 0.1	0.3
Diazepam	1.9 ± 0.2	−0.4	−1.2 – 0.5	0.5
Urethane	2.2 ± 0.4	0	-	-
Gp91phox
Uninterrupted	1.4 ± 0.3	0.1	−0.5 – 0.7	0.9
Diazepam	1 ± 0.1	−0.3	−0.8 – 0	0.2
Urethane	1.4 ± 0.2	0	-	-
BDNF
Uninterrupted	2.9 ± 0.5	−2.6	−4.2 – −0.7	0.03
Diazepam	3.2 ± 0.5	−2.3	−4.2 – −0.6	0.04
Urethane	5.5 ± 0.8	0	-	-

SEM = standard error of mean. P-value is obtained by a Mann-Whitney test comparing diazepam and uninterrupted to urethane.