Figure 3.

miR-182 is upregulated locally in the aIC following novel taste consumption in a GABA_AR-mediated, muscarinic AChR-dependent manner. a, QR2 pre-mRNA levels were unchanged in the aIC of mice following novel taste. b, miR-182 expression was measured 1 and 3 h following familiar or novel taste consumption in the aIC of mice. miR-182 expression was elevated 1 h, but not 3 h, following novel taste. c, Rats were given intraperitoneal injections of scopolamine or vehicle 1 h—and then aIC infusions of vehicle or bicuculline 20 min—before familiar taste. Animals were killed 1 h later, and miR-182 was measured. d, miR-182 expression was significantly increased in the aIC after 1 h, in rats that received bicuculline locally to the aIC and vehicle intraperitoneally. e, Infusions of vehicle or Eserine then were given locally to the aIC of rats 20 min before familiar taste. Animals were killed 1 h later, and miR-182 was measured. f, miR-182 expression significantly increased in rat aICs that received Eserine locally, compared with vehicle. g, One of four predicted miR-182 seeds to QR2 mRNA binding sites (miR-182, green; QR2 mRNA, red) in the rat. Data are shown as the mean ± SEM. *p < 0.05; **p < 0.01. See supporting data in Extended Data Figure 3-1 and Extended Data Figure 3-2.