Figure 7.

Absence of angiogenic response after VEGF delivery. Confocal images through the abducens nucleus following double immunofluorescence against GLUT-1 (a marker of endothelial cells used to label blood vessels; B, E, H) and ChAT (a marker of motoneurons; A, D, G) in control (A–C), axotomy (D–F), and after VEGF application (G–I). The merged images of GLUT-1 and ChAT immunostaining are shown for each situation in C, F, I, respectively. Note the similarity in the density of blood vessels between control, axotomy, and VEGF intraventricular injection. The genu of the facial nerve is indicated (VIIg). Scale bars: 200 μm in C (for A–C), 200 μm in F (for D–F), and 200 μm in I (for G–I).