Figure S5.

G–V-Ca²⁺ relations with predictions from Scheme 1 using parameters from Fit B, with adjustable parameter D. (A) G–V-Ca²⁺ relations in the absence of NS11021 bathed in 0 µM (orange triangle), 1 µM (green circle), 10 µM (blue square), and 100 µM Ca²⁺ (black diamond). Parameters are listed under Fit B in Table 1. Lines represent the predictions with D = 16. (B–E) G–V-Ca²⁺ relations in 0.1, 1, 10, and 30 µM NS11021, respectively. Lines represent predictions with D = 18 (0.1 µM), 23 (1 µM), 42 (10 µM), and 61 (30 µM). (F) Fitted values for D versus [NS11021]. These suggest that the major effects of NS11021 on G–V-Ca²⁺ relations may be explained in part by an increase in D with increasing [NS11021] (but see more detailed explanation in Results).