Table 2. Results of changing individual parameters in Scheme 1 to account for effects of NS11021.

Parameter	Fit A		Fit B
	Value at 30 µM NS11021	χ2	Value at 30 µM NS11021	χ2
J0	0.32	1.96	0.36	2.53
KD (µM)	6.8	11.8	2.9	11.29
L0	8.7 × 10−5	2.16	2.1 × 10−4	3.77
zJ (e0)	0.82	11.35	1.3	9.14
zL (e0)	1.8	11.52	2.0	12.09
C	6.0	10.40	35	11.66
D	74	2.37	61	1.56
E	5.0	9.46	5.1	9.86

Simultaneous (global) fitting was performed using G–V relations acquired over a range of Ca²⁺ for [NS11021] at 0, 0.1, 1, 10, and 30 µM, with parameters shown above adjusted individually, as described in Materials and methods. Adjusted parameter values at 30 µM NS11021 are shown above, along with summed χ² values for fitting over the entire range of [NS11021] and [Ca²⁺] (based on 410 experimental data points). Unadjusted parameter values (corresponding to 0 µM NS11021) can be found in Table 1. The bold values emphasize the parameters L₀, J₀, and D that, when changed, provided nearly equal descriptions of the experimental G-V-Ca²⁺ relations.