Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 28;152(6):e201912499. doi: 10.1085/jgp.201912499

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Suárez-Delgado et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 8. — Mutations at V381 in the W366F background produce permanently inactivated channels. (A) Representative traces of gating currents recorded from the expressed channels indicated in each panel. Currents were elicited by voltage-clamp pulses of 60-ms duration ranging from −110 to 30 mV in 10-mV steps from a holding potential of −100 mV. (B) Normalized voltage dependence of charge movement (Q–V) of the three mutants plotted (following its color code from A) and compared with the voltage dependence of the peak-normalized conductance of the background mutation W366F (cyan rhombuses). Q–V data were fitted to Eq. 5. The number of experiments were W366F/V381A, n = 13; W366F/V381T, n = 5; and W366F/V381I, n = 9. (C) The time course of current decay of the three double mutants is plotted as a function of pulse voltage. ON-gating current decay was fitted to a single exponential to determine the time constant. Number of experiments is as in B. Data in B and C are mean ± SEM.