Fig. 4.

Aspirin induces apoptosis dependent on Bim elevation. (A) PC‐9GROR cells were performed with osimertinib in indicated doses and time points, and Bim proteins were detected by western blot analysis. (B) PC‐9GROR and H1975‐OR cells were transfected with siRNA, and the Bim level was detected by western blot analysis. (C) The cell viability was detected with MTT assay after knocking down Bim expression in PC‐9GROR and H1975‐OR cells. Histogram showing the IC₅₀ values for the indicated groups. *P < 0.05 compared with the siNC + osimertinib group; ^† P < 0.05 compared with the siBim + osimertinib group. (D) The cell proliferation was detected by the Ki67 proliferation assay after knocking down Bim expression in PC‐9GROR and H1975‐OR cells. (E) The apoptotic cells were measured by flow cytometry analysis after knocking down Bim expression in PC‐9GROR and H1975‐OR cells. DAPI, 4′,6‐diamidino‐2‐phenylindole; NC, negative control.