Fig. 2.

m6A modification catalyzed by METTL3 and METTL14 increases LNCAROD stability in SCC cells. (A) The mRNAs levels of METTL3 and METTL14 are elevated in HNSCC according to TCGA dataset. (B) Correlation analysis of LNCAROD with METTL3 or METTL14 in HNSCC samples. (C) RIP‐PCR assays performed by using m6A antibody suggested that LNCAROD is m6A modified in HK1 cell (n = 3 per group). (D) Depletion either METTL3 or METTL14 led to reduction of m6A modification level of LNCAROD (n = 3 per group). (E) Silencing either METTL3 or METTL14 resulted in downregulation of LNCAROD level in HK1 cells (n = 3 per group). (F) Pulse‐chase assay suggested that silencing METTL3 and METTL14 shortened the half‐life of LNCAROD. All data are mean ± SD. Data were analyzed by using Student's t‐test. **P < 0.01, ***P < 0.001.