Fig. 3.

NCS1 and NFκB‐regulated genes are differentially expressed in human breast cancer tissue compared to normal breast tissue. (A) Expression heat map of differentially expressed (P < 0.05) NFκB‐regulated genes in normal (n = 5) versus cancerous breast tissue (n = 8). NFκB‐related genes show distinct expression patterns between groups, indicating altered NFκB‐signaling in human breast cancer. A color legend is pictured with a scale from −2 to + 2‐fold change. (B) Gene expression values of NCS1 mRNA were plotted in normal (n = 5) versus cancerous breast tissue (n = 8) and revealed increased NCS1 gene expression in human breast cancer compared to normal breast tissue. **P < 0.01, determined by Student's t‐test. RNA microarray analysis was performed as described previously (Harvell et al., 2013). Data were obtained from the Gene Expression Omnibus (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE31192).