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. 2020 Jun 2;15(6):e0232549. doi: 10.1371/journal.pone.0232549

Fig 1. Prolonged incubation of S. marcescens 1912768R and isolation of mutants with prodigiosin biosynthesis defects.

Fig 1

(A) Changes in the number of all viable strains and percentage of emerged white strains during the prolonged incubation of WT. (B) Frequency of de novo emergence of prodigiosin-defective mutants. R1-10 represent ten single colonies of WT 1912768R cultured independently under the same culture conditions. (C) Representative images of the WT strain 1912768R and the two mutants. (D) Quantification of prodigiosin production in LB medium. *, P < 0.05; ***, P< 0.001.